alexa Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Kim JK, Mulrooney SB, Hausinger RP

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Abstract Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.
This article was published in J Bacteriol and referenced in Journal of Microbial & Biochemical Technology

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