alexa Biotreatment and bioassessment of heavy metal removal by sulphate reducing bacteria in fixed bed reactors.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Cruz Viggi C, Pagnanelli F, Cibati A, Uccelletti D, Palleschi C,

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Abstract In this work a batch-optimised mixture (w/w \%: 6\% leaves, 9\% compost, 3\% Fe(0), 30\% silica sand, 30\% perlite, 22\% limestone) was investigated in a continuous fixed bed column reactor for the treatment of synthetic acid-mine drainage (AMD). A column reactor was inoculated with sulphate-reducing bacteria and fed with a solution containing sulphate and heavy metals (As(V), Cd, Cr(VI), Cu and Zn). At steady state, sulphate abatement was 50+/-10\%, while metals were totally removed. A degradation rate constant (k) of 0.015+/-0.001h(-1) for sulphate removal was determined from column data by assuming a first order degradation rate. Reduction of AMD toxicity was assessed by using the nematode Caenorhabditis elegans as a test organism. A lethality assay was performed with the toxicants before and after the treatment, showing that only 5\% of the animals were still alive after 48h in presence of the contaminants, while the percentage increased to 73\% when the nematodes were exposed to the solution eluted from the column. This article was published in Water Res and referenced in Journal of Bioremediation & Biodegradation

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