alexa Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers.
Pulmonology

Pulmonology

Journal of Clinical Respiratory Diseases and Care

Author(s): Friedenstein AJ, Chailakhyan RK, Gerasimov UV

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Abstract Fibroblast colonies (clones) were obtained by explantation of bone marrow single-cell suspensions and were used to establish multicolony and single-colony derived fibroblast cultures by successive passaging of either pooled or individual colonies. When transplanted in diffusion chambers after 20-30 cell doublings in vitro, the descendants of fibroblast colony-forming cells (FCFC), whether grown from single or pooled colonies, retained the ability for bone and cartilage formation. The content of osteogenic precursors in the cultured progeny significantly outnumbered the initiating FCFC. Thus the high proliferative potential of bone marrow FCFC and their ability to serve as common precursors of bone and cartilage-forming cells makes them probable candidates for the role of osteogenic stem cells.
This article was published in Cell Tissue Kinet and referenced in Journal of Clinical Respiratory Diseases and Care

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