alexa Cadaveric bone marrow and spleen cells for transplantation.
Surgery

Surgery

Journal of Transplantation Technologies & Research

Author(s): Sderdahl G, Tammik C, Remberger M, Ringdn O

Abstract Share this page

Abstract Cadaveric bone marrow was harvested from 20 brain-dead donors to determine optimal conditions for procurement for transplantation. The number of nucleated cells obtained from 1 ml of bone marrow was significantly higher in vertebrae (87+/-20 x 10(6), mean +/- s.e.m.) than in the sternum (10.2+/-3.8 x 10[6]) or ribs (4.9+/-2.0 x 10[6]). Viability of cells was not significantly affected by storage temperature (4 degrees C or 20 degrees C) or duration of storage (6-72 h). In addition to bone marrow, spleen cells were harvested from three cadaveric donors. The mean yield from 1 g of spleen tissue was 4.4 x 10(6) nucleated cells. Using magnetic beads, we removed 96\% of T lymphocytes without affecting the total yield of stem cells from cadaveric bone marrow. Using CD34-positive cell selection, we obtained a 99.6\% T cell depletion efficiency, but with a loss of 60\% of CD34-positive cells. Using optimized techniques, we obtained an estimated mean yield of 5.5 x 10(10) mononuclear cells from the whole thoracic and lumbar vertebral column. With a mean fraction of CD34-positive cells of 2.1+/-0.3\%, recovery and purity were not affected by site of sample, temperature or donor age. In contrast, the CD34-positive fraction in spleen preparations was 0.41+/-0.06\%. When analyzing the number of colony-forming units (CFU-GM, BFU-E and CFU-GEMM), we found no significant differences between cadaveric bone marrow and bone marrow aspirates from living donors. However, cells harvested from the spleen gave significantly fewer CFUs than did bone marrow from living donors. We conclude that bone marrow from cadaveric donors can be harvested and procured with a high degree of viability and good function. With an appropriate technique of harvesting and procurement, it seems feasible to recover enough stem cells for transplantation. This article was published in Bone Marrow Transplant and referenced in Journal of Transplantation Technologies & Research

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version