alexa Carnosine and beta-alanine release is stimulated by glutamatergic receptors in cultured rat oligodendrocytes.
Toxicology

Toxicology

Journal of Clinical Toxicology

Author(s): Bakardjiev A

Abstract Share this page

Abstract Oligodendrocytes obtained from rat brain 0-2 A progenitor cells and differentiated in culture take up beta-alanine and synthesize carnosine (beta-Ala-His). The present study was designed to determine whether carnosine and beta-alanine are released from such cultures in response to some stimuli. An evoked release of these substances was not observed when the cells were incubated with 1 mM glutamate or 0.3 mM kainate. Addition of 0.1 mM cyclothiazide (CTZ) to the corresponding stimulus was accompanied by a distinct peak of release consisting of both carnosine and beta-alanine. The efflux was blocked completely in the case of kainate and to 80\% in the case of glutamate when 50 microM 6,7-dinitroquinoxaline-2,3 (1H,4H)-dion (DNQX) was added to the cells at the same time as the receptor agonist. An increase of the efflux was observed in the presence of Zn2+. This effect was concentration-dependent. Total substitution of NaCl in the efflux medium by LiCl caused only a partial reduction of the release. GABA or 55 mM KCl showed only negligible effect. A large release of carnosine and beta-alanine was observed when oligodendrocyte cultures were treated with Ca2+ ionophore A 23187. These results suggest that oligodendrocytes exhibit a glutamate receptor-mediated release of carnosine and beta-alanine. The release is dependent on elevated intracellular Ca2+ concentration.
This article was published in Glia and referenced in Journal of Clinical Toxicology

Relevant Expert PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords