Author(s): Porrero MC, Mentaberre G, Snchez S, FernndezLlario P, CasasDaz E,
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Abstract The presence of methicillin-susceptible Staphylococcus aureus (MSSA) was analyzed in different free-living wild animals to assess the genetic diversity and predominant genotypes on each animal species. Samples were taken from the skin and/or nares, and isolates were characterized by spa typing, multilocus sequence typing (MLST) and antimicrobial susceptibility testing. The proportion of MSSA carriers were 5.00, 22.93, 19.78, and 17.67\% in Eurasian griffon vulture, Iberian ibex, red deer, and wild boar, respectively (P = 0.057). A higher proportion of isolates (P = 0.000) were recovered from nasal samples (78.51\%) than skin samples (21.49\%), but the 9.26\% of red deer and 18.25\% of wild boar would have been undetected if only nasal samples had been tested. Sixty-three different spa types were identified, including 25 new spa types. The most common were t528 (43.59\%) in Iberian ibex, t548 and t11212 (15.79\% and 14.04\%) in red deer, and t3750 (36.11\%) in wild boar. By MLST, 27 STs were detected, of which 12 had not been described previously. The most frequent were ST581 for Iberian ibex (48.72\%), ST425 for red deer (29.82\%), and ST2328 for wild boar (42.36\%). Isolates from Eurasian griffon vulture belong to ST133. Host specificity has been observed for the most frequent spa types and STs (P = 0.000). The highest resistance percentage was found against benzylpenicillin (average, 22.2\%), although most of the S. aureus isolates were susceptible to all antimicrobial tested. Basically, MSSA isolates were different from those MRSA isolates previously detected in the same animal species. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
This article was published in Appl Environ Microbiol
and referenced in Pharmaceutica Analytica Acta