alexa Cell-free circulating DNA: Diagnostic value in patients with renal cell cancer.
Genetics

Genetics

Molecular Biology: Open Access

Author(s): Hauser S, Zahalka T, Ellinger J, Fechner G, Heukamp LC, , Hauser S, Zahalka T, Ellinger J, Fechner G, Heukamp LC,

Abstract Share this page

Abstract OBJECTIVES: To analyse the diagnostic and prognostic value of cell-free DNA in patients with renal cell carcinoma (RCC). PATIENTS AND METHODS: Cell-free DNA was measured in 35 patients with RCC and 54 healthy individuals using quantitative real-time PCR. ACTB-106 detects fragmented cell-free DNA due to apoptosis and ACTB-384 detects long DNA fragments by necrosis. DNA-Integrity (ACTB-384/ACTB-106 ratio) served as measure of DNA fragmentation. RESULTS: Levels of both DNA fragments were increased in RCC patients compared to healthy individuals (ACTB-384: 1.77 vs. 0.61ng/ml, p=0.0003; ACTB-106: 1.31ng/ml vs. 0.77 ng/ml p=0.003). Receiver operator characteristic analysis (ROC) showed at a threshold level of 1.03 ng/ml for ACTB-106 68.6\%, sensitivity and 70.4\% specificity (AUC: 0.69). ROC analysis showed at a threshold level of 1.70 ng/ml for ACTB-384 57.1\%, sensitivity and 81.5\% specificity (AUC: 0.73). DNA integrity was increased in RCC (1.07 vs. 0.72 p=0.04). In vascular invasion the DNA integrity was reduced (p=0.003). CONCLUSION: Cell-free-DNA levels are increased in RCC. The DNA integrity indicates mostly necrotic origin in RCC.
This article was published in Anticancer Res and referenced in Molecular Biology: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords