alexa Cell-interactive 3D-scaffold; advances and applications.
Biomedical Sciences

Biomedical Sciences

Journal of Biomedical Engineering and Medical Devices

Author(s): Dutta RC, Dutta AK

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Abstract Culturing cells ex vivo that differentiate and maintain in vivo characteristics holds great promise not only for the pragmatic revelations of cell function but also in tissue engineering and regenerative medicine. Lack of de-novo extra-cellular matrix (ECM) milieu, which plays a crucial role in generating physical and chemical signals besides providing structural support is attributed to be the major hurdle in normal cell growth in vitro. Hence, to comprehend the outcome of cell biology research in clinical context, it is important that the cell culture based models should incorporate both the three dimensional (3D) organization and multi cellular complexity of an organ while allowing experimental interventions in a desirable manner. This calls for the development of ECM-mimicking 3D scaffold, which can be integrated with relevant ECM cues to offer cell interactive versatility for different medical and non-medical applications. Present review discusses the status of ECM mimicking for 3D cell culture and its diverse implications. This article was published in Biotechnol Adv and referenced in Journal of Biomedical Engineering and Medical Devices

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