alexa Cellular image analysis and imaging by flow cytometry.
Genetics

Genetics

Single Cell Biology

Author(s): Basiji DA, Ortyn WE, Liang L, Venkatachalam V, Morrissey P

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Abstract Imaging flow cytometry combines the statistical power and fluorescence sensitivity of standard flow cytometry with the spatial resolution and quantitative morphology of digital microscopy. The technique is a good fit for clinical applications by providing a convenient means for imaging and analyzing cells directly in bodily fluids. Examples are provided of the discrimination of cancerous from normal mammary epithelial cells and the high-throughput quantitation of fluorescence in situ hybridization (FISH) probes in human peripheral blood mononuclear cells. The FISH application will be enhanced further by the integration of extended depth-of-field imaging technology with the current optical system.
This article was published in Clin Lab Med and referenced in Single Cell Biology

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