alexa Characterization and Some Cultural Conditions of a Cyclodextrin Glycosyltransferase-producing Alkalophilic Bacillus sp.


Journal of Food & Industrial Microbiology

Author(s): Nobuyuki Nakamura, Koki Horikoshi

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A cyclodextrin glycosyltransferase-producing bacterium was isolated from soil in a high alkaline pH medium containing 1% of Na2CO3. The isolated alkalophilic microorganism was identified as a member of the genus Bacillus. Highest yield of the enzyme was obtained by using the following medium; 1% soluble starch, 5% corn steep liquor, 0.1% K2HPO4, 0.02% MgSO4·7H2O and 1% Na2CO3. The crude enzyme showed a very broad pH-activity curve and had two optimum pH ranges at 4.5-5.0 and 7.5-8.5. The crude enzyme also catalyzed the formation of cyclodextrins at both of the optimum pH ranges.

This article was published in Agricultural and Biological Chemistry and referenced in Journal of Food & Industrial Microbiology

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