alexa Characterization of betaines using electrospray MS MS.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Wood KV, Bonham CC, Miles D, Rothwell AP, Peel G,

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Abstract Betaines are an important class of naturally occurring compounds that function as compatible solutes or osmoprotectants. Because of the permanent positive charge on the quaternary ammonium moiety, mass spectrometric analysis has been approached by desorption methods, including fast atom bombardment and plasma desorption mass spectrometry. Here we show that electrospray ionization MS gives comparable results to plasma desorption MS for a range of authentic betaine standards and betaines purified from plant extracts by ion exchange chromatography. A distinct advantage of electrospray ionization MS over plasma desorption MS is the capability of obtaining product ion spectra via MS/MS of selected parent ions, and hence structural information to discriminate between ions of identical mass.
This article was published in Phytochemistry and referenced in Journal of Chromatography & Separation Techniques

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