alexa Characterization of proanthocyanidins from grape seeds.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Gabetta B, Fuzzati N, Griffini A, Lolla E, Pace R,

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Abstract Leucoselect (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex LH-20 resin and analysis of the isolated fractions by gel permeation chromatography (GPC) and electrospray mass spectrometry (ESI-MS) led to the complete characterization of the proanthocyanidin constituents of Leucoselect. The analysis revealed the presence of approximately 15\% of (+)-catechin (1) and (-)-epicatechin (2), 80\% of (-)-epicatechin 3-O-gallate (3), dimers, trimers, tetramers and their gallates and 5\% of pentamers, hexamers, heptamers and their gallates.
This article was published in Fitoterapia and referenced in Journal of Chromatography & Separation Techniques

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