alexa Characterization of the unique organization and co-regulation of a gene cluster required for phenol and benzene catabolism in Pseudomonas sp. M1.
Environmental Sciences

Environmental Sciences

Journal of Petroleum & Environmental Biotechnology

Author(s): Santos PM, SCorreia I

Abstract Share this page

Abstract This work describes a new genetic organization and co-regulation of a cluster of genes involved in the first steps of phenol and benzene catabolic pathways in Pseudomonas sp. M1, different from the established models for Pseudomonas upper pathway. Pseudomonas sp. M1 was isolated by others from the sediments of the Rhine River and exhibits an exceptional biodegradation ability towards a wide range of toxic and/or recalcitrant compounds. Although the taxonomic classification of strain M1 could not be determined, we found in a previous study that Pseudomonas citronellolis is the closest species. The genetic organization characterized in this study, the phc (phenol catabolism) genes, includes eight clustered genes, encoding a catechol 1,2-dioxygenase (phcA), a multicomponent phenol hydroxylase (phcKLMNOP) and the transcriptional regulator PhcR (phcR). PhcR controls the transcription of the referred seven clustered genes from two catabolic promoters: Pa (for phcA) and Pk (for phcKLMNOP). In agreement with in silico prediction, the activity of Pa and Pk promoters was proved to depend on the presence of sigma(54). Both promoters are phenol and benzene inducible and evidence supporting the unique sigma(54)-dependent co-regulation of the phenol/benzene inducible genes phcA and phcKLMNOP, mediated by PhcR, was obtained. This article was published in J Biotechnol and referenced in Journal of Petroleum & Environmental Biotechnology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version