alexa Chitosan microspheres as immobilized dye affinity support for catalase adsorption.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Molecular and Genetic Medicine

Author(s): Shentu J, Wu J, Song W, Jia Z

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Abstract Chitosan microsphere (CS) was prepared by phase-inversion method as the support matrices. Cibacron Blue F3GA (CB) was covalently attached to the chitosan microspheres, and thus the novel dye-affinity adsorbent was obtained. These Cibacron Blue F3GA-attached chitosan microspheres (CB-CS) were used in the catalase (CAT) adsorption studies. The maximum CAT adsorption capacity of Cibacron Blue F3GA-attached chitosan microspheres was 28.4 mg/g at pH 7.0. Langmuir adsorption model was found to be applicable in interpreting CAT adsorption. Significant amount of the adsorbed CAT (up to 90.6\%) was eluted in the elution medium containing 0.5 M NaSCN at pH 8.0. It appears that CB-CS can be applied for adsorption of CAT without causing any denaturation. This article was published in Int J Biol Macromol and referenced in Journal of Molecular and Genetic Medicine

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