alexa CLARITY for mapping the nervous system.
Biochemistry

Biochemistry

Bioenergetics: Open Access

Author(s): Chung K, Deisseroth K

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Abstract With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies. This article was published in Nat Methods and referenced in Bioenergetics: Open Access

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