alexa Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies.


Journal of Transplantation Technologies & Research

Author(s): Donnenberg AD, Gorantla VS, Schneeberger S, Moore LR, Brandacher G,

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Abstract AIMS: Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand-forelimb transplants performed at the University of Pittsburgh (PA, USA). MATERIALS & METHODS: The cell separation and release testing were performed at the University of Pittsburgh Cancer Institute's Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation of Cellular Therapy (FACT) and for clinical flow cytometry by the College of American Pathologists (CAP).
This article was published in Regen Med and referenced in Journal of Transplantation Technologies & Research

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