alexa [Cloning of PsPIP1 gene from Polygonum sibiricum Laxm. and analy-sis of its expression in response to NaHCO3].
Agri and Aquaculture

Agri and Aquaculture

Journal of Aquaculture Research & Development

Author(s): Wang Y, Liu GJ, Yan XF, Yang CP, Liu MK,

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Abstract Gene PsPIP1 (GenBank accession No. EU626398) containing a complete ORF was obtained using rapid amplification of cDNA ends (RACE) from the cDNA library of Polygonum sibiricum Laxm. leaves. The length of cDNA was 1 004 bp, which encoded a peptide of 285 amino acid residues. Based on other kinds of plant aquaporin amino acid sequences, the phylogenetic evolution, and tertiary structure of protein comparison, this gene was classified into aquaporin subfamily. Expression analysis by Real-time PCR showed that PsPIP1 gene was expressed in leaves, stems, and underground stems. The expression level of PsPIP1 gene was higher in leaves than in underground stems and was the lowest in stems. The expression pattern of PsPIP1 gene induced by NaHCO3 stress and de-stressing also varied remarkably.
This article was published in Yi Chuan and referenced in Journal of Aquaculture Research & Development

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