alexa Collagen implants and cortico-spinal axonal growth after mid-thoracic spinal cord lesion in the adult rat.
Materials Science

Materials Science

Journal of Nanomedicine & Biotherapeutic Discovery

Author(s): Joosten EA, Br PR, Gispen WH

Abstract Share this page

Abstract We describe an experimental model to study regeneration of lesioned corticospinal tract (CST) fibers in the adult rat spinal cord. After transection of all CST fibers at mid-thoracic level the gap is grafted with a sterile, cell-free collagen matrix. Two methods of collagen-application are used: 1) injection of a fluid collagen solution into the lesioned area which self-assembles in situ and 2) implantation of a solid collagen gel. At 4 weeks post-implantation CST axons are anterogradely labelled with horseradish-peroxidase (HRP). The collagen implant is evaluated for ingrowth of CST axons. The histopathological reaction (gliotic response) around the lesion and within the matrix is also studied. After application of a fluid collagen solution into the lesion area HRP-labelled CST axons can be visualized within the implant. In addition, astroglial and reactive microglial cells invade the collagen-matrix. On the other hand, if collagen is implanted as an already self-assembled gel, no ingrowth of labelled CST axons nor of astroglial/reactive microglial cells is observed. Both methods of collagen-application result in a considerable reduction of the gliotic response as compared to the ungrafted animals. We conclude that the method of application of collagen (i.e., fluid or gel) considerably affects the response of lesioned CST axons. The application of a fluid collagen graft which in situ self-assembles is beneficial for the regrowth of lesioned CST axons in rat spinal cord. In this respect the formation of an astroglial scaffolding structure within the (fluid) collagen, probably due to optimal integration between host and graft, is very important. The inability of injured CST fibers to enter the solid collagen graft may be related to the absence of an astroglial scaffolding structure within the implant. This article was published in J Neurosci Res and referenced in Journal of Nanomedicine & Biotherapeutic Discovery

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords