alexa Commercially available versus home produced egg media for isolation of mycobacteria.


Journal of Microbial & Biochemical Technology

Author(s): Harris G, Rayner A, Watt B

Abstract Share this page

Abstract AIMS: To compare the growth of clinical isolates of mycobacteria on in-house and commercial egg media. METHODS: Fresh test media were inoculated with dilutions of the test organisms and growth, colonial morphology and microscopic appearance were compared blindly by two observers. The process was repeated after the test media had been stored for three months. The user friendliness of each of the test media was also assessed. RESULTS: There was no difference in the microscopic appearance of any given mycobacterial species between different media. All of the test media grew the test species, although Mycobacterium bovis required four weeks on BioMerieux media, compared with two weeks on the other media. There was little obvious effect of storage on any of the media, except with M kansasii. Individual species gave characteristic colonial appearances on inhouse media; all of the commercial media gave non-specific colonial appearances that made presumptive identification very difficult. There were clear differences in the user friendliness of different media. CONCLUSIONS: Although commercially available egg media in general gave good growth of mycobacteria, only in-house media combined good growth with useful colonial features.
This article was published in J Clin Pathol and referenced in Journal of Microbial & Biochemical Technology

Relevant Expert PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version