alexa Comparative evaluation of ELISAs based on ten recombinant or purified Leishmania antigens for the serodiagnosis of Mediterranean visceral leishmaniasis.
Immunology

Immunology

Journal of Clinical & Cellular Immunology

Author(s): Maalej IA, Chenik M, Louzir H, Ben Salah A, Bahloul C,

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Abstract This study compared a panel of 10 enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of Mediterranean visceral leishmaniasis (MVL). The ELISAs were based on either one of the following Leishmania antigens: crude soluble Leishmania antigens (SLAs), recombinant (r) antigens (namely: rgp63, rK39, gene B protein, r H2A and r H2B histones proteins, rLACK, rPSA-2, r P20) and purified lipophosphoglycan. Most of the test antigens showed good performance (sensitivity > 85\%, specificity > 80\%). rK39 and SLA-based ELISA gave the best results in terms of sensitivity (100\%) and predictive value of the negative (100\%). The best specificity (97\%) and the best predictive value of the positive (92\%) were obtained with rK39. These results show that several Leishmania antigens are suitable to design a diagnostic ELISA of MVL. However, recombinant proteins add little to the classic crude SLA, which still represents a very good and less costly alternative.
This article was published in Am J Trop Med Hyg and referenced in Journal of Clinical & Cellular Immunology

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