alexa Comparative proteomics of rat liver and Morris hepatoma 7777 plasma membranes.
Pharmaceutical Sciences

Pharmaceutical Sciences

Journal of Bioequivalence & Bioavailability

Author(s): Clifton JG, Li X, Reutter W, Hixson DC, Josic D

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Abstract Plasma membranes from normal rat liver and hepatocellular carcinoma Morris hepatoma 7777 were selectively solubilized by use of different reagents. After selective solubilization, proteins were identified by nano-HPLC-electrospray ionization tandem mass spectrometry (LC-ESI MS/MS). Using simple software, the patterns of proteins identified in membrane solubilizates from liver and hepatoma were compared. Proteins identified in Morris hepatoma 7777 and not in the corresponding membrane solubilizate from liver, mostly members of the annexin and heat shock protein families, are discussed as potential candidate markers for hepatocellular carcinomas. This article was published in J Chromatogr B Analyt Technol Biomed Life Sci and referenced in Journal of Bioequivalence & Bioavailability

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