Author(s): Font de Valdez G, Savoy de Giori G, Pesce de Ruiz Holgado A, Oliver G
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Abstract Cultures of 14 lactic acid bacteria species were freeze-dried in 10 or 20\% non-fat skim milk and in distilled water containing one of the following additives: bovine albumin, glycogen, dextran, polyethylene glycol (PEG) 1000, PEG 4000, PEG 6000, glycerol, beta-glycerophosphate, sodium glutamate, asparagine, or cysteine. Each of the potential protective agents tested exhibited marked variations in the protection afforded to different species, none of them was effective for the preservation of viability of thermophilic lactobacilli. However, glycerol provided effective protection for L. leichmannii ATCC 4797 (90\% survival), while L. bulgaricus ATCC 11842 reached a viability of 78\% with 0.04 M cysteine.
This article was published in Cryobiology
and referenced in Journal of Bioprocessing & Biotechniques