Author(s): Strachan AJ, Evans NE, Williams OM, Spencer RC, Greenwood R,
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Abstract This study set out to validate the Hs27 ReadyCell assay (RCCNA) as an alternative CCNA method compared against a commonly used commercial enzyme immunoassay (EIA) method and toxigenic culture (TC) reference standard. A total of 860 samples were identified from those submitted to the Health Protection Agency microbiology laboratories over a 30-week period. RCCNA performed much better than EIA when using TC as a gold standard, with sensitivities of 90.8\% versus 78.6\% and positive predictive value of 87.3\% to 81.9\%, respectively. The Hs27 Human Foreskin Fibroblast ReadyCells are an easy-to-use and a sensitive CCNA method for the detection of toxigenic Clostridium difficile directly from stool. A turnaround time of up to 48 h for a negative result and possible need for repeat testing make it an unsuitable method to be used in most clinical laboratory setting. Copyright © 2013 Elsevier Inc. All rights reserved.
This article was published in Diagn Microbiol Infect Dis
and referenced in Internal Medicine: Open Access