alexa Comparison of a laboratory-developed RT-PCR and the CDC RT-PCR protocol with rapid immunodiagnostic testing during the 2009 H1N1 influenza A pandemic.


Clinical Depression

Author(s): Liao RS, Landt O, Hill JT

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Abstract We evaluated the performance of a laboratory-developed multiplex real-time reverse transcription-PCR assay (LDT rRT-PCR), the Centers for Disease Control and Prevention (CDC) 2009 H1N1 rRT-PCR protocol using the LightCycler 480 II, the multiplex xTAG Respiratory Virus Panel (xTAG RVP), and rapid immunodiagnostic testing (RIDT) using the BinaxNOW Influenza A & B to detect 2009 H1N1 with 426 nasopharyngeal swab specimens during the 2009 H1N1 pandemic. The specificity of the methods tested was ≥98\%, and the individual test sensitivities were RIDT at 42.3\% [95\% confidence interval (CI), 31.4-54.0], LDT rRT-PCR at 98.9\% (95\% CI, 92.9-99.9), CDC 2009 H1N1 rRT-PCR at 78.2\% (95\% CI, 67.8-86.0), and xTAG RVP at 93.1\% (95\% CI, 85.0-97.2). A negative RIDT result should not be used to make decisions with respect to treatment or infection prevention. rRT-PCR is the preferred first-line diagnostic test for detecting 2009 H1N1 influenza A. Copyright © 2011 Elsevier Inc. All rights reserved. This article was published in Diagn Microbiol Infect Dis and referenced in Clinical Depression

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