alexa Comparison of a nested polymerase chain reaction--restriction fragment length polymorphism method, the PATH antigen detection method, and microscopy for the detection and identification of malaria parasites.


Journal of Antivirals & Antiretrovirals

Author(s): Parkes R, Lo T, Wong Q, IsaacRenton JL, Byrne SK

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Abstract A nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, the PATH antigen detection method, and light microscopy were compared for their capacity to detect and identify Plasmodium species. One hundred and thirty-six blood specimens obtained from patients suspected of having malaria were examined by each of the three methods. Forty-four specimens were positive for malaria using microscopy as the "gold standard". The sensitivity for nested PCR was 100\%, and the specificity was 98\%. For the detection of Plasmodium falciparum, the antigen detection method had a sensitivity of 100\% and a specificity of 97\%. Species identification obtained using PCR-RFLP was identical or superior to light microscopy in 42 cases (96\%). Although the nested PCR-RFLP method was more sensitive and specific, the rapid turnaround time and high sensitivity of the antigen detection method makes it a useful adjunct to standard microscopy.
This article was published in Can J Microbiol and referenced in Journal of Antivirals & Antiretrovirals

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