alexa Comparison of in vitro (chicken-skin membrane) versus in vivo (live hamster) blood-feeding methods for maintenance of colonized Phlebotomus papatasi (Diptera: Psychodidae).
Clinical Research

Clinical Research

Journal of Clinical Research & Bioethics

Author(s): Rowton ED, Dorsey KM, Armstrong KL

Abstract Share this page

Abstract An in vitro feeding method using chicken-skin membranes and human blood was compared with an established in vivo method using anesthetized hamsters for blood-feeding mass-reared phlebotomine sand flies. Parameters measured were percentage of sand flies taking blood meals, number of eggs laid per female, and percentage of eggs that hatched. Females from a long established (>20 yr) colony of Phlebotomus papatasi (Scopoli) from Israel landed on and started feeding sooner on the hamster than on the membrane. However, when sand flies were allowed access to the membrane feeder for the same length of time as the anesthetized hamster, the feeding percentages were not significantly different and were usually better on the membrane feeder if flies were allowed access for a longer time. Egg production and percent hatch between the two feeding methods were not statistically different. Based on these results, we conclude that the chicken-skin membrane feeding method is a viable alternative to the use of live animals for feeding large numbers of P. papatasi.
This article was published in J Med Entomol and referenced in Journal of Clinical Research & Bioethics

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords