Author(s): Shokeir A, Osman Y, ElSherbiny M, Gabr M, Mohsen T,
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Abstract OBJECTIVE: To determine whether acellular matrix could be used for partial urethral replacement and to compare regeneration over acellular matrix versus normal spontaneous urethral regeneration. MATERIALS AND METHODS: The study included 21 male mongrel dogs in which a 3-cm segment including half of the urethral circumference was excised. In 13 dogs (study group), the defect was covered by acellular matrix of the same length and width obtained from female mongrel dogs and prepared to have complete cell lysis with keeping of the fiber framework. In 8 dogs (control group), the urethral defect was not covered by any urethral tissue. In both groups, an 8F feeding tube was kept inside the urethra for a mean duration of 2 weeks. In the study group, dogs were sacrificed at 1 week, 2 weeks, 3 weeks and then one dog every month for 10 months. In the control group, one dog was sacrificed every month for 8 months. RESULTS: All dogs survived the procedure. In the study group, 10 dogs underwent urethrogram; 8 were normal, 1 had diverticulum and 1 had relative narrowing. In the control group, 6 dogs underwent urethrogram; 5 were normal and 1 showed relative narrowing. Histopathological examination of the study group showed gradual regeneration over the acellular matrix with normal appearance at 20 weeks. In the control group, normal healing was observed at 2 months and thereafter. CONCLUSION: Regeneration of all components of the urethra can occur gradually over acellular matrix and is complete at 20 weeks. Regeneration of a urethral defect 3-cm long including half of the urethral lumen is possible with or without acellular matrix.
This article was published in Eur Urol
and referenced in Journal of Bioengineering & Biomedical Science