Author(s): Xiong XC, Li WL, Li X, Xing JM, Liu HZ
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Abstract Several bacteria, Bacillus brevis R-6, Pseudomonas delafleldii R-8, Nocardia globerula R-9, Bacillus sphaericus R-16, Rhodococcus erythropolis LSSE8-1 and Gordonia nitida LSSEJ-1, which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate, were investigated. Desulfurization products were quantitively determined by HPLC. Result revealed that each of these bacteria desulfurize DBT at a different rate. In order to obtain more information, the fragments encoding desulfurizing enzymes were studied. Desulfurization genes of R-6 and R-8 were separately amplified via PCR with specific primers based on the related sequences of Rhodococcus sp. IGTS8. Both sequences areminimally 99\% related to IGTS8 sequence. Afterwards, dsz operon of LSSEJ-1 and R-9 were amplified and cloned. Sequences are also highly conservative. Data shows that identity of dszA between R-9 and IGTS8 is 99.6\%, and identity of dszA between LSSEJ-1 and IGTS8 is 99.9\%; dszB sequence of R-9 and LSSEJ-1 is 99.6\% similarity to their counterpart sequence from IGTS8;Identity of dszC between R-9 and IGTS8 is 99.9\%, and identity of dszC between LSSEJ-1 and IGTS8 is 99.1\% . It may be deduced that the origins of desulfurization genes from mesophilic bacteria are the same.
This article was published in Wei Sheng Wu Xue Bao
and referenced in Industrial Engineering & Management