alexa Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry.
Chemical Engineering

Chemical Engineering

Mass Spectrometry & Purification Techniques

Author(s): Saffert RT, Cunningham SA, Mandrekar J, Patel R

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Abstract We evaluated 3 preparatory methods for processing positive blood culture bottle broths prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis-differential centrifugation, 10\% sodium dodecyl sulfate (SDS), and the Sepsityper kit. Initial evaluation used genus and species level cutoff scores of 1.700-1.999 and ≥ 2.000, respectively. Processing of an initial 79 blood bottle cultures by differential centrifugation yielded correct identifications of 51 (65\%) to the genus and 34 (43\%) to the species levels. One hundred and one different blood bottles were simultaneously processed with 10\% SDS and Sepsityper. Both yielded genus-level identifications for 77 (76\%), and the 2 yielded species-level identifications for 49 (49\%) and 54 (54\%) of bottles, respectively. Adjustment of the score cutoff criteria for genus (1.500-1.699) and species (≥ 1.700) improved identification percentages, particularly for species-level identifications (P < 0.05). Copyright © 2012 Elsevier Inc. All rights reserved. This article was published in Diagn Microbiol Infect Dis and referenced in Mass Spectrometry & Purification Techniques

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