alexa Complex Formation of SMAP KAP3, a KIF3A B ATPase Motor-associated Protein, with a Human Chromosome-associated Polypeptide
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Proteomics & Bioinformatics

Author(s): Kazuya Shimizu, Hiromichi Shirataki, Tomoyuki Honda, Seigo Minami, Yoshimi Takai

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We have recently isolated SMAP (Smg GDS-associated protein; Smg GDS:small G protein GDPdissociation stimulator) as a novel Smg GDS-associated protein, which has Armadillo repeats and is phosphorylated by Src tyrosine kinase. SMAP is a human counterpart of mouse KAP3 (kinesin superfamily-associatedprotein) that is associated with mouse KIF3A/B (akinesin superfamily protein), which functions as a microtubule-based ATPase motor for organelle transport. We isolated here a SMAP-interacting protein from a human brain cDNA library, identified it to be a human homolog of XenopusXCAP-E ( X enopus chromosome-associated polypeptide), a subunit of condensins that regulate the assembly and structural maintenance of mitotic chromosomes, and named it HCAP (Human chromosome-associatedpolypeptide). Tissue and subcellular distribution analyses indicated that HCAP was ubiquitously expressed and highly concentrated in the nuclear fraction, where SMAP and KIF3B were also present. SMAP was extracted as a ternary complex with HCAP and KIF3B from the nuclear fraction in the presence of Mg-ATP. The results suggest that SMAP/KAP3 serves as a linker between HCAP and KIF3A/B in the nucleus, and that SMAP/KAP3 plays a role in the interaction of chromosomes with an ATPase motor protein.

This article was published in J Biol Chem and referenced in Journal of Proteomics & Bioinformatics

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