alexa Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans.


Journal of Allergy & Therapy

Author(s): Arancia S, Sandini S, Cassone A, De Bernardis F, La Valle R

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Abstract A method for detection of Candida albicans in biological samples (blood, serum, urine) was developed by the use of polymerase chain reaction (PCR) amplification of a DNA fragment of a gene coding for a 65 kDa mannoprotein of C. albicans (CaMP65). The PCR amplifies a 220 bp fragments whose specificity for C. albicans was demonstrated by Southern blot with a non-radioactive probe, leading to the differentiation from all other yeast species or human and bacterial DNA. The sensitivity of this assay was 5-10 C. albicans cells per milliliter of biological sample. This article was published in Mol Cell Probes and referenced in Journal of Allergy & Therapy

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