Author(s): Temple GF, Dozy AM, Roy KL, Kan YW
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Abstract A human tRNALys gene was converted to an amber suppressor by site-specific mutagenesis of the anticodon. The mutated tRNALys gene directed synthesis of a tRNA that suppressed the UAG amber nonsense mutation in beta O thalassemia mRNA. Such genes may be used to detect other nonsense mutations in mammalian cells and may provide an approach to gene therapy for beta O thalassaemia due to nonsense mutations.
This article was published in Nature
and referenced in Journal of Molecular and Genetic Medicine