alexa Continuous automated measurement of carbon dioxide produced by microorganisms in aerobic conditions: application to proteic film biodegradation.
Microbiology

Microbiology

Research & Reviews: Journal of Microbiology and Biotechnology

Author(s): Annick Manceau

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A new automated set-up based on the original Sturm test was developed for determining the biodegradability of proteic mulching films. This method is based on the measurement of released (respiratory metabolism of microorganisms) carbon dioxide to determine film biodegradability. Maintenance of constant aerobic conditions in thermoregulated bioreactors and continuous measurement of released CO2 with an infrared differential gas analyser were the main advantages of this set-up. It was verified with a plasticised proteic film that soil extracts could be used as microbial sources instead of activated sludge that was usually used in laboratory biodegradation tests. To cite this article: S. Lefaux et al., C. R. Chimie 7 (2003).RésuméUn nouveau dispositif de mesure basé sur le principe du test de Sturm a été mis au point et adapté à la quantification de la biodégradation de films protéiques destinés au paillage agricole. Ce montage expérimental permet de déterminer la biodégradabilité potentielle d'un film polymère en mesurant le dioxyde de carbone respiratoire dégagé lors de la dégradation du matériau testé par des micro-organismes. Ce dispositif est équipé d’un analyseur différentiel à infrarouge, qui mesure en continu le CO2 dégagé de six réacteurs thermorégulés. Les expériences réalisées en conditions aérobies sur un film protéique plastifié ont montré que la microflore du sol pouvait être retenue comme inoculum bactérien à la place des boues activées habituellement utilisées dans les tests de laboratoire. Pour citer cet article : S. Lefaux et al., C. R. Chimie 7 (2003).

This article was published in C. R. Chimie and referenced in Research & Reviews: Journal of Microbiology and Biotechnology

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