Author(s): Shrestha NK, Hadano S, Kamachi T, Okura I
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Abstract Because Nitrosomonas europaea contains ammonia-oxidizing enzyme, nitrite reductase, and nitrous oxide reductase, the conversion of ammonia to dinitrogen was tried with different reaction conditions. In aerobic reaction conditions, ammonium was converted to nitrite (NO2-), while under oxygen-limiting or oxygen-free conditions, NO2(-)-N formed from ammonia oxidation by N. europaea was reduced to N2O and dinitrogen with 22\% conversion. During denitrification, optimal pH for the production of N2O and dinitrogen was found to be 7.0-8.0. Dinitrogen was not produced in acidic pH <7.0. A low partial oxygen pressure as well as oxygen-free conditions are favorable for high production of dinitrogen.
This article was published in Appl Biochem Biotechnol
and referenced in Journal of Bioremediation & Biodegradation