alexa Copper-free click chemistry for dynamic in vivo imaging.
Medicine

Medicine

Advanced Techniques in Biology & Medicine

Author(s): Baskin JM, Prescher JA, Laughlin ST, Agard NJ, Chang PV,

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Abstract Dynamic imaging of proteins in live cells is routinely performed by using genetically encoded reporters, an approach that cannot be extended to other classes of biomolecules such as glycans and lipids. Here, we report a Cu-free variant of click chemistry that can label these biomolecules rapidly and selectively in living systems, overcoming the intrinsic toxicity of the canonical Cu-catalyzed reaction. The critical reagent, a substituted cyclooctyne, possesses ring strain and electron-withdrawing fluorine substituents that together promote the [3 + 2] dipolar cycloaddition with azides installed metabolically into biomolecules. This Cu-free click reaction possesses comparable kinetics to the Cu-catalyzed reaction and proceeds within minutes on live cells with no apparent toxicity. With this technique, we studied the dynamics of glycan trafficking and identified a population of sialoglycoconjugates with unexpectedly rapid internalization kinetics.
This article was published in Proc Natl Acad Sci U S A and referenced in Advanced Techniques in Biology & Medicine

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