alexa Correlation and quantitation of microRNA aberrant expression in tissues and sera from patients with breast tumor.
Genetics

Genetics

Molecular Biology: Open Access

Author(s): Wang F, Zheng Z, Guo J, Ding X, Wang F, Zheng Z, Guo J, Ding X

Abstract Share this page

Abstract OBJECTIVES: MicroRNAs (miRNAs) have been underlined as a promising potential biomarker for breast cancer but are limited to tissue specimens. Clinical specimens of sera are more abundant and more conveniently collected than tissues. This work was designed to investigate the expression and correlation of a selected panel of miRNAs associated with breast tumor in tissues and matching serum samples. METHODS: Tumor tissues, adjacent non-tumor tissues and matching serum samples were collected from 68 patients with newly diagnosed breast tumors. Normal control sera were collected from 40 healthy subjects. A panel of 6 miRNAs (miRNA-21, 106a, 126, 155, 199a and 335) were selected and their aberrant expression levels were quantified by using real-time PCR technique. RESULTS: A high correlation of miRNA expression level was found between breast tumor tissues and sera. MiR-21, miR-106a and miR-155 were significantly over-expressed in the tumor specimens compared with those in normal controls (P < 0.05), whereas miR-126, miR-199a and miR-335 were significantly under-expressed (P < 0.05). Furthermore, the relative expression of miR-21, miR-126, miR-155, miR-199a and miR-335 was closely associated with clinicopathologic features of breast cancer (P < 0.05), such as histological tumor grades and sex hormone receptor expression. CONCLUSIONS: Selective expression and modulation of miRNAs could be potential blood-based biomarkers for breast cancer diagnosis, grading and prognosis. Our results should encourage further studies on the use of miRNAs in serum samples as an easy and convenient method of breast cancer screening. Copyright © 2010 Elsevier Inc. All rights reserved. This article was published in Gynecol Oncol and referenced in Molecular Biology: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords