alexa Cost-effectiveness of a modified two-step algorithm using a combined glutamate dehydrogenase toxin enzyme immunoassay and real-time PCR for the diagnosis of Clostridium difficile infection.
General Science

General Science

Journal of Forensic Research

Author(s): Vasoo S, Stevens J, Portillo L, Barza R, Schejbal D, , Vasoo S, Stevens J, Portillo L, Barza R, Schejbal D,

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Abstract The analytical performance and cost-effectiveness of the Wampole Toxin A/B EIA, the C. Diff. Quik Chek Complete (CdQCC) (a combined glutamate dehydrogenase antigen/toxin enzyme immunoassay), two RT-PCR assays (Progastro Cd and BD GeneOhm) and a modified two-step algorithm using the CdQCC reflexed to RT-PCR for indeterminate results were compared. The sensitivity of the Wampole Toxin A/B EIA, CdQCC (GDH antigen), BD GeneOhm and Progastro Cd RT-PCR were 85.4\%, 95.8\%, 100\% and 93.8\%, respectively. The algorithm provided rapid results for 86\% of specimens and the remaining indeterminate results were resolved by RT-PCR, offering the best balance of sensitivity and cost savings per test (algorithm ∼US$13.50/test versus upfront RT-PCR ∼US$26.00/test). Copyright © 2012. Published by Elsevier B.V. This article was published in J Microbiol Immunol Infect and referenced in Journal of Forensic Research

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