alexa Coupled transcription and translation within nuclei of mammalian cells.
Biochemistry

Biochemistry

Biochemistry & Physiology: Open Access

Author(s): Iborra FJ, Jackson DA, Cook PR

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Abstract It is widely assumed that the vital processes of transcription and translation are spatially separated in eukaryotes and that no translation occurs in nuclei. We localized translation sites by incubating permeabilized mammalian cells with [3H]lysine or lysyl-transfer RNA tagged with biotin or BODIPY; although most nascent polypeptides were cytoplasmic, some were found in discrete nuclear sites known as transcription "factories." Some of this nuclear translation also depends on concurrent transcription by RNA polymerase II. This coupling is simply explained if nuclear ribosomes translate nascent transcripts as those transcripts emerge from still-engaged RNA polymerases, much as they do in bacteria. This article was published in Science and referenced in Biochemistry & Physiology: Open Access

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