Author(s): Tollner TL, VandeVoort CA, Overstreet JW, Drobnis EZ
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Abstract Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20\% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5\% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computer-aided analysis of sperm motion was used to determine the percentage motility (\%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the \% of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of \% M and of \% viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3\% glycerol level gave superior results to 5\% glycerol for \%M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.
This article was published in J Reprod Fertil
and referenced in Cloning & Transgenesis