alexa Current HIV-2 diagnostic strategy overestimates HIV-2 prevalence in China.
Infectious Diseases

Infectious Diseases

Journal of AIDS & Clinical Research

Author(s): Qiu M, Liu X, Jiang Y, Nkengasong JN, Xing W, , Qiu M, Liu X, Jiang Y, Nkengasong JN, Xing W,

Abstract Share this page

Abstract A significant number of HIV-2 infections have been reported in China using Western blot as per current guidelines for HIV-2 diagnosis in China. However, most specimens were also positive on HIV-1 Western blot suggesting cross-reactivity and possible overestimation. We carried out the current study to evaluate a strategy to diagnose the HIV-2 infections in China. A total of 119 specimens received from 16 provinces were likely to be HIV-2 when tested according to current guidelines in China using the Genelabs Western blot (HIV Blot 2.2 WB). Further testing by HIV-2 WB (Bio-Rad New LAV Blot II or Genelabs HIV Blot 1.2 WB) scored 56 (47.1\%) of 119 samples with banding pattern suggestive of HIV-2 infection, and 63 (52.9\%) were HIV-2 indeterminate. A peptide-based HIV-1 and HIV-2 enzyme immunoassay for differential diagnosis of HIV-1 and HIV-2 infections was validated and used. This in-house EIA demonstrated that only 1 (0.8\%) of 119 specimens had HIV-2 specific antibodies, while 2 (1.7\%) were dually reactive. These results were highly concordant (>99\%) with those by Inno-LIA HIV-I/II (Innogenetics, Belgium), which also use specific peptides for type-specific diagnosis. Our data demonstrates that HIV-2 infection is rare in China, and HIV-2 Western blot may overestimate the prevalence of HIV-2 in the population with HIV-1. The HIV-2 diagnostic strategy in China needs to be revised to include more specific peptide-based immunoassays. Copyright 2009 Wiley-Liss, Inc. This article was published in J Med Virol and referenced in Journal of AIDS & Clinical Research

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 5th International Conference on HIV/AIDS, STDs and STIs
    November 13-14, 2017 Las Vegas, Nevada, USA
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords