alexa Current technologies to identify protein kinase substrates in high throughput.
Biochemistry

Biochemistry

Journal of Glycobiology

Author(s): Xue L, Tao WA

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Abstract Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.
This article was published in Front Biol (Beijing) and referenced in Journal of Glycobiology

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