alexa Cysteine-rich 61 (CYR61) is up-regulated in proliferative diabetic retinopathy.


Journal of Clinical & Cellular Immunology

Author(s): Zhang X, Yu W, Dong F

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Abstract BACKGROUND: To investigate the role of CYR61 as a retinal angiogenic factor in proliferative diabetic retinopathy (PDR). METHODS: Effects of CYR61 on RF/6A cell proliferation, migration and angiogenesis were observed by MTT assay, Transwell assay, and tube formation assay. The expression and distribution of CYR61 on retina layers of diabetic mouse were demonstrated by immunohistochemistry. The expression of Cyr61 mRNA in diabetic mouse retina was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Vitreous CYR61 levels of PDR and non-diabetic patients were measured by enzyme-linked immunosorbent assay (ELISA). Expression and distribution of CYR61 on epiretinal membrane of PDR, proliferative vitreoretinopathy (PVR) and idiopathic epiretinal membrane were evaluated by immunohistochemistry. RESULTS: RF/6A cell proliferation, migration and tube formation capacity increased with increased concentration of CYR61 (p = 0.000). Anti-CYR61 antibody could inhibit cell migration and tube formation promoted by CYR61. In diabetic mouse, CYR61 was expressed in retina layers just as normal mouse, but the staining was stronger than normal in ganglion cell layer and inner plexiform layer. The Cyr61 mRNA expression in retina of diabetic mouse was more than that in normal mouse (p = 0.009). Vitreous CYR61 level was higher in patients with PDR than non-diabetic patients (p = 0.000). PDR patients with plenty of neovasculature on retina and epiretinal membranes had higher level of vitreous CYR61 than patients with little neovasculature (p = 0.001). CYR61 expressed in the cytoplasm of epiretinal membranes in PDR, especially in the wall cells of the tube-like structure. CONCLUSIONS: CYR61 are likely to be involved in the pathogenesis of diabetic retinopathy, and may play a role in the course of neovasculation. This article was published in Graefes Arch Clin Exp Ophthalmol and referenced in Journal of Clinical & Cellular Immunology

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