Author(s): Brown SM, Khanani AM, Xu KT
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Abstract PURPOSE: To determine the individual variability of the dark-adapted pupil diameter over 2 testing sessions using a standardized dark-adaptation protocol. SETTING: Texas Tech University Health Sciences Center, Lubbock, Texas, USA. METHODS: In this prospective observational cohort study, 40 volunteers with no history of ocular disease, surgery, or injury other than requirement for refractive correction were included. The right eye was tested. A standardized dark-adaptation protocol was used that controlled for accommodation and patient alertness. Infrared, still digital photographs were taken after 5 and 10 minutes of dark adaptation and analyzed independently by 2 investigators using digital-image software. Two test sessions were performed 1 to 7 days apart. Lifestyle factors such as sleep, diet, and exercise were not controlled. RESULTS: The mean subject age was 31.5 years (range 20 to 49 years). There were 20 men; 27 subjects wore correction for myopia, and 13 wore no correction. The mean interval between test sessions was 2 days (range 1 to 7 days). The mean difference and 95\% confidence intervals for pupil diameter difference between sessions were as follows: 5-minute readings, +0.032 mm (-0.030 to +0.094); 10-minute readings, -0.006 mm (-0.059 to +0.047); mean of 5- and 10-minute readings, +0.013 mm (-0.038 to +0.064). Using the paired t test, the pupil diameter did not differ significantly between sessions in 5-minute dark adaptation (P =.2980), 10-minute dark adaptation (P =.8263), or the mean (P =.6049). CONCLUSION: Using a consistent dark-adaptation protocol that controlled for alertness, individuals aged 20 to 49 years showed no significant variation in dark-adapted pupil diameter when tested twice in 1 week.
This article was published in J Cataract Refract Surg
and referenced in Journal of Proteomics & Bioinformatics