Author(s): Bassaris HP, Lianou PE, Skoutelis AT, Papavassiliou JT, Phair JP, Bassaris HP, Lianou PE, Skoutelis AT, Papavassiliou JT, Phair JP
Abstract Share this page
Abstract The effects of sera obtained from 18 patients with homozygous beta-thalassemia on the adherence and chemotaxis of normal polymorphonuclear leukocytes (PMNLs) was studied. The adherence of PMNLs suspended in thalassemic serum was 3.1\% +/- 2.5\% (mean +/- SD) compared with 17.9\% +/- 8.0\% adherence in control serum (P less than 0.00001). Zymosan-treated thalassemic serum did not generate chemotactic factors, and zymosan-treated normal serum did not induce chemotaxis of PMNLs suspended in thalassemic serum. When added to zymosan-treated normal plasma, thalassemic serum inhibited augmentation of PMNL adherence to nylon columns. These abnormalities were due to a heat-labile factor(s) in the sera of patients with thalassemia. Suspending PMNLs in heated thalassemic serum (56 C for 30 min) resulted in an increase of adherence to 14.6\% +/- 6.2\% (P less than 0.0001). PMNLs suspended in heated thalassemic serum responded normally to chemotactic attractants.
This article was published in J Infect Dis
and referenced in Journal of Blood Disorders & Transfusion