alexa Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments.


Advancements in Genetic Engineering

Author(s): Jasin M, Schimmel P

Abstract Share this page

Abstract Deletion of an essential gene in Escherichia coli was accomplished by transformation of linear DNA fragments that have a Kanr gene segment flanked by sequences homologous to closely spaced regions on the E. coli chromosome. Selection for a double crossover within homologous sequences can effectively delete an entire gene. Cell viability is maintained by provision of the essential gene on a plasmid with a temperature-sensitive replicon, resulting in cells which have a temperature-sensitive phenotype.
This article was published in J Bacteriol and referenced in Advancements in Genetic Engineering

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • International Conference on Epigenetics 2017
    November 13-15, 2017 Frankfurt, Germany
  • International Conference on Genetic Counseling and Genomic Medicine
    February 12-13, 2018 Madrid, Spain

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version