alexa Deoxyribonucleic acid base composition and homology studies of Leptospira.
Microbiology

Microbiology

Journal of Bacteriology & Parasitology

Author(s): Haapala DK, Rogul M, Evans LB, Alexander AD

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Abstract Four distinct genetic groups of leptospiras were demonstrated among selected pathogenic and "biflexa" serological types. Pathogenic leptospiras could be divided into two groups on the basis of per cent guanine + cytosine (GC) in their deoxyribonucleic acid (DNA). One group had 36 +/- 1\%, the other 39 +/- 1\%. The biflexa strains had DNA of 39 +/- 1\% GC, but were further separated into two groups on the basis of DNA-annealing tests. Strains within groups had a high degree of specific duplex formation (75\% binding or more with reference to the homologous DNA). There was little or no genetic relatedness between strains of the four groups (less than 10\% DNA homology). The thermal elution midpoint of heterologous DNA duplexes was always lower than the homologous reaction. The serological relationships among strains were not meaningful in terms of relatedness determined by specific duplex formation.
This article was published in J Bacteriol and referenced in Journal of Bacteriology & Parasitology

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