alexa Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells.
Engineering

Engineering

Journal of Biosensors & Bioelectronics

Author(s): Durand F, StinesChaumeil C, Flexer V, Andr I, Mano N

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Abstract We report for the first time a soluble PQQ-glucose dehydrogenase that is twice more active than the wild type for glucose oxidation and was obtained by combining site directed mutagenesis, modelling and steady-state kinetics. The observed enhancement is attributed to a better interaction between the cofactor and the enzyme leading to a better electron transfer. Electrochemical experiments also demonstrate the superiority of the new mutant for glucose oxidation and make it a promising enzyme for the development of high-performance glucose biosensors and biofuel cells. Copyright © 2010 Elsevier Inc. All rights reserved. This article was published in Biochem Biophys Res Commun and referenced in Journal of Biosensors & Bioelectronics

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