alexa Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells.


Journal of Biosensors & Bioelectronics

Author(s): Durand F, StinesChaumeil C, Flexer V, Andr I, Mano N

Abstract Share this page

Abstract We report for the first time a soluble PQQ-glucose dehydrogenase that is twice more active than the wild type for glucose oxidation and was obtained by combining site directed mutagenesis, modelling and steady-state kinetics. The observed enhancement is attributed to a better interaction between the cofactor and the enzyme leading to a better electron transfer. Electrochemical experiments also demonstrate the superiority of the new mutant for glucose oxidation and make it a promising enzyme for the development of high-performance glucose biosensors and biofuel cells. Copyright © 2010 Elsevier Inc. All rights reserved. This article was published in Biochem Biophys Res Commun and referenced in Journal of Biosensors & Bioelectronics

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version