alexa Detection by immunofluorescence assay of Bartonella henselae in lymph nodes from patients with cat scratch disease.


Journal of Bacteriology & Parasitology

Author(s): Rolain JM, Gouriet F, Enea M, Aboud M, Raoult D

Abstract Share this page

Abstract Laboratory diagnosis of Bartonella henselae infections can be accomplished by serology or PCR assay on biopsy samples. The purpose of our work was to assess immunofluorescence detection (IFD) in lymph node smears using a specific monoclonal antibody directed against B. henselae and a commercial serology assay (IFA) compared with PCR detection. Among 200 lymph nodes examined from immunocompetent patients, 54 were positive for B. henselae by PCR, of which 43 were also positive by IFD. Among the 146 PCR-negative lymph nodes, 11 were positive by IFD. Based on PCR results, the specificity of this new technique was 92.5\%, the sensitivity was 79.6\%, and the positive predictive value was 79.6\%. At a cutoff titer of 64, the sensitivity of the IFA was 86.8\% and the specificity was 74.1\%. Diagnosis of cat scratch disease (CSD) may be improved, with a specificity of 100\%, when the two tests (IFD and IFA) were negative; the sensitivity was 97.4\% if one of the two tests was positive. Since PCR-based detection with biopsy samples is available only in reference laboratories, we suggest using IFD coupled with the commercial serology test for the diagnosis of CSD.
This article was published in Clin Diagn Lab Immunol and referenced in Journal of Bacteriology & Parasitology

Relevant Expert PPTs

Relevant Speaker PPTs

  • R. K. Pandey
    Community oriented integrated ecosystem approach for conservation and sustainable management of forest genetic resources: Challenges in biodiversity conservation in natural tropical forests of India
    PPT Version | PDF Version

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version