alexa Detection of anti-aquaporin-4 antibodies in neuromyelitis optica: current status of the assays.


Journal of Addiction Research & Therapy

Author(s): Waters P, Vincent A

Abstract Share this page

Abstract BACKGROUND: Neuromyelitis optica (NMO) is a severe inflammatory demyelinating disease that predominantly affects the optic nerve and spinal cord. Since the discovery of a specific serum marker for NMO in 2004, and its subsequent identification as an antibody to aquaporin-4 (AQP-4), various methods have been developed to test for the antibodies in patients sera. OBJECTIVE: To assess the principal methods used to measure AQP-4 antibodies in patients sera, describe their contribution to neuromyelitis spectrum disease and examine their value in the early detection of disease. METHODS: We compared the published data on each assay and used the relapsing NMO cohort as a uniform patient group for direct assay comparison. RESULTS: The indirect immunofluorescence assay, a cellbased assay (CBA) and a fluorescence-based immunoprecipitation assay have broadly similar high sensitivities (86\%, 91\% and 83\%) in the relapsing cohort, but the indirect immunofluorescence has a lower specificity (91\%) compared with the other two (both 100\% specific). CONCLUSIONS: The indirect immunofluorescence assay for NMO-IgG allows the detection of antibodies in routine screening, but the CBA for AQP-4 antibodies is the most sensitive. The fluoroimmunoprecipitation assay is a potentially high-throughput test for identifying positive sera and for serial estimations of antibody levels, but in its present form is slightly less sensitive. Overall, these assays are proving very useful in helping to diagnose those patients with longitudinally extensive transverse myelitis or recurrent optic neuritis who are likely to have relapsing NMO, including patients with myelopathy and Sjogrens syndrome, but it appears to be less often positive in patients with monophasic NMO.
This article was published in Int MS J and referenced in Journal of Addiction Research & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version