alexa Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry.


Journal of Blood Disorders & Transfusion

Author(s): Khan SS, Solomon MA, McCoy JP Jr

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Abstract The finding of angiogenic and vasculogenic cells in the peripheral circulation may have profound effects on the course of a variety of diseases ranging from cancer to cardiovascular disease. These cells are ascribed to be endothelial in nature and are generally referred to as circulating endothelial cells if mature or as endothelial progenitor cells if immature. Different approaches have been used to detect these cells, including in vitro culture, magnetic bead isolation, and flow cytometry. We review flow cytometric methods for the detection and enumeration of these cells and provide technical suggestions to promote the accurate enumeration of circulating endothelial cells and endothelial progenitor cells. This article was published in Cytometry B Clin Cytom and referenced in Journal of Blood Disorders & Transfusion

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